How to Revive Frozen-thawed Cultures

JCM distributes some fungi to users in the form of frozen-thawed agar disks in cryotubes. These cultures were prepared according to the method of Ito (1993). To revive the cultures, please follow the directions shown below.

Prepare a suitable medium for the fungal strain by consulting the JCM On-line Catalogue of Strains.
Check that the cap of the cryotube is closed firmly, and then wipe the tube with cotton wool containing 70% alcohol.
Open the cap slowly under sterile environment. To prevent contamination, it is recommended that the upper part of the tube is covered with a sterilized cotton sheet or cotton wool (NOT containing alcohol).
Pick up agar disks from the cryotube with a suitable tool (e.g., microspatula, platinum loop, etc.), and inoculate them on the medium.
Incubate the culture under the directed condition for several weeks.

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JCM accession number
JCM:

Example: JCM = 12345 (Exact match search)

Scientific name 1
Genus: Species:

Example 1: Genus = Micromonospora Example 2: Species = aurantiaca Example 3: Genus = %monospo, Species = %ensis (Prefix match search at each box. Inside match or suffix match search can be performed by using "%" as a wild-card character like Example 3. Case-insensitive)

Scientific name 2
Scientific name:

Example 1: Scientific name = Micromonospora chalcea Example 2: Scientific name = Lactobacillus paracasei subsp. paracasei (Prefix match search. Inside match or suffix match search can be performed by using "%" as a wild-card character. Case-insensitive)

Culture medium
JCM Medium No.: Name of Medium:

Example 1: JCM Medium No. = 58 Example 2: Name of Medium = malt extract (Exact match search for "JCM Medium No." and inside match search for "Name of Medium". Case-insensitive)