How to Revive Lyophilized or L-Dried Cultures

Please follow the steps below to open the ampoule and revive the microbial strain. The rehydration solution, medium and culture conditions specified for the strain are listed in our online catalogue and should be checked before starting. Please note that the shape of the ampoule delivered may vary depending on the production period.
https://jcm.brc.riken.jp/en/catalogue_e

Keep the ampoule on a flat place, and make a file-cut at the neck.

Using a sterile Pasteur pipette, add 0.3 to 0.5 ml of a suitable rehydration solution* into the ampoule.
*See the JCM On-line Catalogue at:
JCM On-line Catalogue

Wipe the ampoule with cotton wool containing 70% ethanol.

Inoculate the entire suspension on the specified medium for the strain described in our online catalogue and incubate under the specified conditions.

How to Revive Lyophilized or L-Dried Cultures (PDF 270KB)

Cover the ampoule with a sterile cotton sheet, and cut it carefully at the neck. Do not use a cotton sheet containing alcohol.

Frequently Asked Questions (FAQ)
https://jcm.brc.riken.jp/en/ordering_e_faq#culture

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JCM accession number
JCM:

Example: JCM = 12345 (Exact match search)

Scientific name 1
Genus: Species:

Example 1: Genus = Micromonospora Example 2: Species = aurantiaca Example 3: Genus = %monospo, Species = %ensis (Prefix match search at each box. Inside match or suffix match search can be performed by using "%" as a wild-card character like Example 3. Case-insensitive)

Scientific name 2
Scientific name:

Example 1: Scientific name = Micromonospora chalcea Example 2: Scientific name = Lactobacillus paracasei subsp. paracasei (Prefix match search. Inside match or suffix match search can be performed by using "%" as a wild-card character. Case-insensitive)

Culture medium
JCM Medium No.: Name of Medium:

Example 1: JCM Medium No. = 58 Example 2: Name of Medium = malt extract (Exact match search for "JCM Medium No." and inside match search for "Name of Medium". Case-insensitive)