Notice: Preservation of a wrong strain of Lysinibacillus fusiformis JCM 12229T and replacement with the authentic strain

December 14, 2015

Lysinibacillus fusiformis (formerly Bacillus fusiformis) JCM 12229T was accessioned from NRRL* (USA) in 2003 and had been distributed since 2004. Recently, we found that JCM 12229T was identified as Bacillus megaterium by the 16S rRNA gene sequence analysis during the course of quality test of JCM strains. We obtained the strain of Lysinibacillus fusiformis from NRRL again and confirmed its authenticity. We prepared the new stock using the authentic culture. Now, it is available upon request.

Since 2011, we have examined DNA sequences of the PCR-amplified 16S rRNA gene of newly deposited strains and compared the sequences with those provided by depositors of the strains to confirm the authenticity of the cultures. But, for the strains deposited before 2011 such as this case, we did not confirm the authenticity by sequencing of the 16S rRNA gene at the time of their deposition. Now, we are checking the authenticity of the strains that have not yet been confirmed by gene sequencing. We appreciate your support and understanding of our activities.

We informed users of JCM 12229T of this matter in detail with our sincere apology, asked for stopping its use and started providing the replacement.

If you have any question and concern on this matter, please feel free to contact us (

*NRRL; Northern Regional Research Laboratory, now Agricultural Research Service (ARS) Culture Collection, US Department of Agriculture, USA 

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