JCM On-line Catalogue of Strains

 Scientific name 1
 Genus:
 Species:
    Example 1: Genus = Micromonospora
Example 2: Species = aurantiaca
Example 3: Genus = %monospo, Species = %ensis
(Prefix match search at each box. Inside match or suffix match search can be performed by using "%" as a wild-card character like Example 3. Case-insensitive)

 Scientific name 2
 Scientific name:
    Example 1: Scientific name = Micromonospora chalcea
Example 2: Scientific name = Lactobacillus paracasei subsp. paracasei
(Prefix match search. Inside match or suffix match search can be performed by using "%" as a wild-card character. Case-insensitive)

 JCM accession number
   JCM:
    Example: JCM = 12345 (Exact match search)

 Accession number of other culture collection
 Acronym:
 Number:
    Example 1: Acronym = DSM, Number = 43067
Example 2: Acronym = VKM, Number = Ac-636
(Exact match search at both boxes. Case-sensitive)

 Keyword
   Keyword:
    Example: Keyword = genome sequence
(Inside match search. Case-insensitive)

 Culture medium
 JCM Medium No.:
 Name of Medium:
    Example 1: JCM Medium No. = 58
Example 2: Name of Medium = malt extract
(Exact match search for "JCM Medium No." and inside match search for "Name of Medium". Case-insensitive)

How to Revive Frozen-thawed Cultures

JCM distributes some fungi to users in the form of frozen-thawed agar disks in cryotubes. These cultures were prepared according to the method of Ito (1993). To revive the cultures, please follow the directions shown below.

  1. Prepare a suitable medium for the fungal strain by consulting the JCM On-line Catalogue of Strains.
  2. Check that the cap of the cryotube is closed firmly, and then wipe the tube with cotton wool containing 70% alcohol.
  3. Open the cap slowly under sterile environment. To prevent contamination, it is recommended that the upper part of the tube is covered with a sterilized cotton sheet or cotton wool (NOT containing alcohol).
  4. Pick up agar disks from the cryotube with a suitable tool (e.g., microspatula, platinum loop, etc.), and inoculate them on the medium.
  5. Incubate the culture under the directed condition for several weeks.

 

Reference :

    Ito, T. 1993. A simple method for the transport of fungal cultures stored by freezing. Bull. JFCC 9: 9-12.